CHALLENGES OF LABORATORY SAMPLING AND DIAGNOSIS OF SARS-COV-2 VIRUS OF DISEASE (COVID-19)

Authors

  • Abedi Elkhichi, Parisa PhD Student in Bacteriology, Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran
  • Asalani Mehr, Masoumeh Medical Microbiology Associate Professor Qazvin University of Medical Sciences Research Center, Qazvin, Iran (Corresponding Author)
Abstract:

Background & Aims: Given the prevalence of SARS-CoV-2 worldwide, it is essential to identify people infected with the virus and determine its different types to control the global outbreak of COVID-19. The results of the studies are controversial, so this study examines these diagnostic challenges, including the types of diagnostic methods, the type and time of sampling, and even the clinical condition of the disease. Materials & Methods: In this systematic review, studies conducted from 2003 to May 2020 in English on the challenges of laboratory sampling and diagnostics of coronaviruses and SARS-CoV-2 virus were reviewed. These articles were obtained by searching for keywords in databases, PubMed, Scopus and Embace as well as Google scholar search engine and duplicate articles were removed from the study. Results: In the initial search, 98 articles were extracted that after eliminating duplicates and evaluating the title and abstract, finally 64 articles had the necessary conditions for review and were included in the study. The results of the studies showed that, although RT-PCR is the gold standard for the diagnosis of Covid-19 disease, but it requires a sufficient concentration of viral RNA in the patient sample for proper evaluation of the sample. Since the concentration of viral RNA in the patient sample is not constant, this can affect the results obtained. Although the best sample to detect the virus in the first days is the nasopharyngeal swab and in the following days, samples taken from the lower respiratory tract, but also numerous samples such as throat swabs and feces can also be used for identification. However, the percentage of viral RNA isolation in these samples is significantly reduced. Conclusion: Due to the global prevalence of Covid-19 disease, early diagnosis of patients, quarantine and timely treatment are of great importance in controlling the epidemic. The use of correct diagnostic tests allows physicians to perform immediate interventions for patients. Although molecular diagnosis is the best method, studies show that it is better not to be satisfied with the results of an experiment and to use a combination of different methods and tests to solve diagnostic challenges.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

A review of coronaviruses focusing on SARS-CoV-2 (2019-nCoV) the cause of COVID-19

Coronaviruses, enveloped positive single-stranded RNA viruses, have become significant pathogens for humans and other vertebrates since the outbreak of SARS (severe acute respiratory syndrome) in 2002 in China. These viruses could infect the respiratory tract of humans and other animals. The outbreaks of SARS and MERS (Middle East respiratory syndrome), which Bats are thought to be the origin o...

full text

A Brief Review on the Advancement of the Molecular and Serological Diagnosis of SARS-CoV-2

The novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has introduced as causative agent for coronavirus disease 2019 (COVID-19) since the 2019 December. Human coronaviruses are classified in Nidovirales order and Coronavirdiae family. This family includes four genera. The SARS-CoV-2 is a member of Betacoronavirus genera and Sarbecovirus linage (linage B). There is ...

full text

Serology Testing for SARS-CoV-2: Benefits and Challenges

As COVID-19 was declared as a pandemic by the World Health Organization (WHO) in March 2020, it is an emerging need to discuss different aspects of this pandemic. In any pandemic, valid and rapid laboratory diagnostic tests are critically important for early diagnosis, which will increase the rate of successful treatment and more importantly prevent the spread of the disease.  

full text

SARS-CoV-2 and COVID-19, evidence from a literature review: review article

  SARS-CoV-2 emerging from Wuhan, China is a member of the Coronaviridae family, which has so far infected and killed many people. The SARS-CoV-2 pandemic affected various aspects of life in Iran and Worldwide, and governments have imposed quarantines and travel bans on an unprecedented scale. The virus causes COVID-19, which can spread through close contact with the infected person, contaminat...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 32  issue 3

pages  156- 174

publication date 2021-05

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023